Modifying Enzymes
Modifying Enzymes
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Thermo Scientific™ Esonucleasi III (Exo III)
Digest double-stranded DNA or DNA-RNA hybrids with Exonuclease III is a 3'–>5' exonuclease.
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Thermo Scientific™ Polinucleotide chinasi T4 (T4 PNK)
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.
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Product Type | T4 Polynucleotide Kinase |
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Enzyme | T4 Polynucleotide Kinase |
Compatible Buffer | 10X Reaction Buffer |
Concentrazione | 10 U/μL |
Thermo Scientific™ Klenow Fragment, LC (2 U/μL)
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
Thermo Scientific™ T4 DNA ligasi
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Thermo Scientific™ Frammento di Klenow
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
Product Type | Klenow Fragment |
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Polimerasi | DNA Polymerase I |
Concentrazione | 10 U/μL |
Cytiva Shrimp Alkaline Phosphatase
Per catalizzare la rimozione di gruppi di fosfato di 5 e 3 da DNA, RNA, nucleotidi e proteine
Sorgente | Batteri |
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Product Type | Fosfatasi alcalina |
Purezza | Purificato per omogeneità |
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Product Type | Klenow Fragment |
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Polimerasi | DNA Polymerase I |
Compatible Buffer | Restriction Enzyme, PCR, RT Buffer |
Concentrazione | 5 U/μL |
Thermo Scientific™ T4 DNA ligasi
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
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Product Type | T4 DNA Ligase |
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Enzyme | DNA Ligase |
Compatible Buffer | 10X T4 DNA Ligase Buffer |
Concentrazione | 5 U/μL |
Thermo Scientific™ Fosfatasi alcalina termosensibile FastAP
Catalyze the removal of 5'- and 3'-phosphate groups from DNA, RNA, nucleotides, and proteins using this thermosensitive alkaline phosphatase .
Product Type | Alkaline Phosphatase |
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Enzyme | Phosphatase |
Compatible Buffer | PCR Buffer, Enzyme Buffer |
Concentrazione | 1 U/μL |
Thermo Scientific™ Uracil-DNA glicosilasi (UDG, UNG)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Content And Storage | Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. |
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Product Type | Uracil DNA Glycosylase |
Enzyme | Uracil-DNA Glycosylase |
Compatible Buffer | 10X Reaction Buffer |
Concentrazione | 1 U/μL |
Thermo Scientific™ T4 DNA ligasi
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
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Thermo Scientific™ Klenow Fragment
Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.
Colore | Colorless |
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Content And Storage | -20°C |
Forma | Solid |
Sorgente | E. coli cells with a cloned bacterial Alkaline Phosphatase gene |
Product Type | Alkaline Phosphatase |
Da utilizzare con (applicazione) | DePhosphorylates cloning vector DNA (10 min. at 37°C), fast thermo-inactivation (5 min. at 75°C), Nucleotide degradation prior to sequencing PCR products, active on Nucleotides, Protein dephosphorylation, restriction En |
pH | 7.4 |
Tampone di conservazione | OPTIZYME™ AP Buffer 10X : Tris HCl 100mM (8 pH at 37°C), MgCl2 50mM, KCl 1M, Triton™ X-100 0.2%, BSA 1mg/mL |
Concentrazione | 1 U/μL |
Colore | Colorless |
---|---|
Content And Storage | -20°C |
Forma | Liquid |
Sorgente | E. coli cells with a cloned gene 30 of bacteriophage T4 |
Product Type | T4 DNA Ligase |
Da utilizzare con (applicazione) | Used to clone restriction Enzyme generated DNA fragments, cloning PCR products, ligation of double-stranded Oligonucleotide linkers or adaptors to DNA, site-directed mutagenesis, amplified fragment length polymorphism, ligase-mediated RNA detection, nick |
pH | 7.5 |
Tampone di conservazione | OPTIZYME™ T4 DNA Ligase Buffer 10X: Tris HCl 400mM, MgCl2 100mM, DTT 100mM, ATP 5mM (7.8 pH at 25°C), OPTIZYME™ PEG 4000 Solution 50%: Polyethylene Glycol 50% (w/v) |
Concentrazione | 5 U/μL |